biotinylated goat anti human ccl18 antibody  (R&D Systems)

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: The concentration of CCL18 was evaluated in 39 peritoneal effluents from patients diagnosed with infectious peritonitis. Effluents were collected on the first day of cloudy effluent. Horizontal bars represent mean ± SEM values. No significant differences were found between Gram− ( N = 13) and Gram+ bacteria ( N = 26) infected cases (unpaired t -test).

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Concentration Assay, Bacteria, Infection

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: Serial cytokine concentrations and cell counts in peritoneal effluent during peritonitis episodes. (a) Evolution of CCL18 effluent levels in serial samples from four patients with infectious peritonitis (IP) shown as IP-1 to IP-4. (b) Evolution of IL-6 effluent levels in serial samples from the same patients. (c) Comparison of neutrophils (PMN) and macrophage (MΦ) cell counts in serial effluent samples. Differential cell counts on D26 (IP-2), D22 (IP-3), and D13 (IP-4) were not available given that the total cell counts were <50 cells/ μ l in those samples.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Comparison

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: Kinetics of effluent cell counts and cytokine concentrations during EP. (a–d) CCL18 effluent levels and cell counts in serial effluent samples. (e–h) IL-6 effluent levels and cell counts in serial effluent samples.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques:

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: Cytospin preparations showing effluent cell content in EP. (a) Hematoxylin and eosin staining. (b) Immunocytochemistry showing CCL18 staining of eosinophils. (c) Background staining with nonimmune serum.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Staining, Immunocytochemistry

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: (a) Flow cytometry analysis of peritoneal effluent leukocytes on D6 according to morphological parameters. LΦ: lymphocytes; MΦ: macrophages. (b) Flow cytometry analysis of CD16 expression in peritoneal effluent cells on D6. The cutoff level for positive staining was set according to isotype control background staining. PMN: polymorphonuclear neutrophils. (c) Flow cytometry analysis of CD66b + polymorphonuclear cells isolated from peritoneal effluent cells. (d) RT-PCR of CCL18 gene expression in CD66 + and CD14 + purified peritoneal leukocytes. (e) Quantitative RT-PCR analysis of CCL18 gene expression in CD66+ and CD14+ purified peritoneal leukocytes.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Flow Cytometry, Expressing, Staining, Control, Isolation, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Purification, Quantitative RT-PCR

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: (a–c) Peritoneal macrophages isolated from patients experiencing peritonitis episodes were treated with vehicle or Zemplar in doses equivalent to 10 −8 M and 10 −9 M of paricalcitol during 24 h (a), 48 h (b), or 72 h (c). Equivalent cell cultures were established in the absence of any treatment. The concentration of CCL18 was evaluated in cell-free supernatants, and the production of CCL18 in the presence of vehicle or drug referred to the production in cell culture media alone. Differences between cultures in the presence of paricalcitol or vehicle were analyzed by the Wilcoxon matched pairs rank test. (d) Peritoneal macrophages were treated with paricalcitol as pure substance, vitamin D, or vehicle (ethanol) for 48 h.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Isolation, Concentration Assay, Cell Culture

Journal: BioMed Research International

Article Title: Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

doi: 10.1155/2018/6415892

Figure Lengend Snippet: (a) Peritoneal macrophages from a patient with recent infectious peritonitis were treated with paricalcitol or vehicle for 24 h. The protein concentration of CCL18 was evaluated in cell culture supernatants by ELISA, and CCL18 mRNA levels were estimated by quantitative RT-PCR in the same cultures. (b) Peritoneal macrophages from an uninfected peritoneum were isolated from the peritoneal lavage upon catheter insertion and treated as in (a). (c) Peripheral blood monocytes were pretreated with vehicle (V), 10 −7 M paricalcitol (Pari), or vitamin D3 for 24 h. The cells were treated thereafter with IL-4 for 3 days before RNA extraction and evaluation expression of CCL18. mRNA levels by quantitative RT-PCR. Mean ± SD values from four independent experiments are shown.

Article Snippet: Biotinylated goat anti-human CCL18 (R&D Systems) was added and incubated overnight at 4°C.

Techniques: Protein Concentration, Cell Culture, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Isolation, RNA Extraction, Expressing